Cytochemical methods for the rapid demonstration of infection at the tissue-biomaterial interface

Abstract

Infection can be an extensive problem developing during implantation of an autogenous or prosthetic (biomaterial) device or graft. Although culture techniques are invaluable in identifying the responsible microorganisms, the times required frequently emphasize the need for rapid staining methods which can reduce the classification times from days to hours. Studies in our laboratories over the past few years have resulted in microwave-accelerated stains and in methods developed by Giammara, which enable the rapid study of glass slides and coverslips by electron as well as light microscopy.