Abstract
Layer crystals such as graphite, molybdenite and mica have a variety of uses in electron microscopy when lamella can be easily prepared with clean surfaces, uniform thickness, high crystal perfection and tolerable waviness. The most common use of thin crystals is as a featureless support for high resolution microscopy. Crystals can also be used as phase plates and to reduce radiation damage. Crystals have long been used to display high resolution images and can be used to accurately characterize electron microscope operational parameters.Crystals for any of the uses described above can in principle be prepared by any technique although their usefulness may be severely limited by nonuniform thickness, surface contamination and sparse fields. Crystals prepared by cleavage with pressure sensitive tape give useful supports with simple equipment and require less than 20 minutes of preparation time for the crystal lamellae mounted on one grid.
Publisher
Cambridge University Press (CUP)
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3. Figure 3 was taken with the Madison HVEM supported by NIH grant S-PO7 RR 00570 through the courtesy of H. Ris. The research was supported by NIH grant 1-R01 GM 25443 , by a NIH Fogarty Senior International Fellowship and by an Overseas Fellowship at Churchill College,Cambridge.