Identification of an immune modulation locus utilising a bovine mammary gland infection challenge model
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Published:2018-05
Issue:2
Volume:85
Page:185-192
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ISSN:0022-0299
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Container-title:Journal of Dairy Research
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language:en
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Short-container-title:Journal of Dairy Research
Author:
Littlejohn Mathew D,Turner Sally-Anne,Walker Caroline G,Berry Sarah D,Tiplady Kathryn,Sherlock Ric G,Sutherland Greg,Swift Simon,Garrick Dorian,Lacy-Hulbert S Jane,McDougall Scott,Spelman Richard J,Snell Russell G,Hillerton J Eric
Abstract
Inflammation of the mammary gland following bacterial infection, commonly known as mastitis, affects all mammalian species. Although the aetiology and epidemiology of mastitis in the dairy cow are well described, the genetic factors mediating resistance to mammary gland infection are not well known, due in part to the difficulty in obtaining robust phenotypic information from sufficiently large numbers of individuals. To address this problem, an experimental mammary gland infection experiment was undertaken, using a Friesian-Jersey cross breed F2herd. A total of 604 animals received an intramammary infusion ofStreptococcus uberisin one gland, and the clinical response over 13 milkings was used for linkage mapping and genome-wide association analysis. A quantitative trait locus (QTL) was detected on bovine chromosome 11 for clinical mastitis status using micro-satellite and Affymetrix 10 K SNP markers, and then exome and genome sequence data used from the six F1sires of the experimental animals to examine this region in more detail. A total of 485 sequence variants were typed in the QTL interval, and association mapping using these and an additional 37 986 genome-wide markers from the Illumina SNP50 bovine SNP panel revealed association with markers encompassing the interleukin-1 gene cluster locus. This study highlights a region on bovine chromosome 11, consistent with earlier studies, as conferring resistance to experimentally induced mammary gland infection, and newly prioritises theIL1gene cluster for further analysis in genetic resistance to mastitis.
Publisher
Cambridge University Press (CUP)
Subject
Animal Science and Zoology,General Medicine,Food Science
Reference24 articles.
1. Mapping quantitative trait loci in crosses between outbred lines using least squares;Haley;Genetics,1994
2. Mastitis in cows milked in an automated or conventional milking system in New Zealand;Lopez-Benavides;Proceedings of the New Zealand Society for Animal Production,2006
3. Selection for mastitis resistance in dairy cattle: a review with focus on the situation in the Nordic countries
4. Quantitative Trait Loci Affecting Health Traits in Swedish Dairy Cattle
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