Characteristics of the autolysis of variants ofLactococcus lactissubsp.cremoris

Abstract

SummaryTwo slime-forming strains (T5and MLS96) and a non-slime-forming strain (HA)of Lactococcus lactissubsp.cremoriswere investigated for autolysis and optimal conditions for the autolytic process were developed. The rate of autolysis was maximal in exponential phase cells in 0·01 M-Na phosphate buffer, pH 6·5–7·5, at 30–45 °C. Autolysis of the native exponential phase walls was activated by trypsin and inhibited by lipoteichoic acid and cardiolipin. Decreased trypsin activation was found in intact exponential phase cells and no activation in stationary phase cells.N-acetylmuramylhydrolase action in the autolytic system ofLc. lactissubsp.cremorisstrains was indicated by the progressive release of reducing groups. No amidase or endopeptidase action was found. Great variation in the rate of autolysis of whole cells was observed between the two slime-forming strains. Intact cells of strain T5exhibited decreased autolytic activity, but a higher rate of autolysis of isolated exponential phase walls. Autolysins from strain T5walls exhibited lower hydrolytic activity against sodium dodecyl sulphate-treated walls of the other two strains as compared with strains MLS96 and HA. Quantitative analysis revealed higher protein and phosphorus and lower hexosamine and rhamnose in cell wall preparations of strain T5compared with the other two strains. Results suggest that the decreased rate of autolysis of strain T5cells may at least in part be caused by cell surface components other than cell-wall peptidoglycan. It is proposed that the difference in autolytic characteristics should be used as a criterion when selecting starter strains of lactococci (streptococci).