Abstract
AbstractThe protozoan parasiteTrypanosoma evansiis responsible for causing Surra in a variety of mammalian hosts over a wide geographical area. In the absence of an effective vaccine and increasing resistance to current chemotherapeutic agents, peptidases from the S9 prolyl oligopeptidase family have been identified as potential drug and vaccine targets. In order to understand the function of these peptidases during infection, three null mutant clones for prolyl oligopeptidase (Δpop), prolyl oligopeptidase-like (Δpop-like) and oligopeptidase B (Δopb) were generated inT. evansiRoTat 1.2 parasites and used for infection of mice. Mice inoculated withT. evansi Δpop-likemutants were able to survive longer than other groups of mice inoculated withΔpop,Δopbmutants or wild-type parasites. The regression analysis of plasma from mice-infected over time usingΔpop-likemutants showed stable levels of interleukin-10 (IL-10) (non-significant slope,P= 0·171) and declining IL-1b levels (negative slope,P= 0·04) when compared with the wild-type control that demonstrated increasing levels of IL-10 and IL-1b (P< 0·01 for both). Further analysis using mouse spleen cells in anin vitro24 h incubation assay revealed that the percentage of IL-10 producing CD3 positive cells display significantly lower values when incubated withΔpop-likeparasites than the wild-type clone (P= 0·002). These results suggest that prolyl oligopeptidase-like peptidase may play a role in immune responses duringT. evansiinfections by affecting interleukin concentrations in the host.
Publisher
Cambridge University Press (CUP)
Cited by
1 articles.
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