Author:
Stępińska Urszula,Kuwana Takashi,Olszańska Bożenna
Abstract
SummaryThe expression of nine serotonin (5-HT) receptor transcripts was studied using reverse transcription polymerase chain reaction (RT-PCR) in germ cells, cleavage and gastrulation stages of Japanese quail, and qPCR for 5-HT3and 5-HT4receptors in oocytes and embryos. We show the presence/absence of nine serotonin transcripts known in birds for receptors 5-HT1A, 5-HT1F, 5-HT2B, 5-HT2C, 5-HT3, 5-HT4, 5-HT5A, 5-HT6and 5-HT7Ain avian germ cells and early embryos. The absence of 5-HT3and 5-HT5Ain primordial germ cells and of 5-HT3and 5-HT7Ain sperm is characteristic. All transcripts appeared in oocytes at all stages (except for 5-HT3and 5-HT5Atranscripts) and all were present in cleaving embryos and at gastrulation, except for 5-HT3, which was permanently observed as late as in stage 4. Interestingly, 5-HT3and 5-HT5Areceptors accumulated in 3-mm and F1 oocytes but were degraded at ovulation and started to be re-transcribed in cleavage stage II embryos and beyond. The selective appearance of 5-HT receptors in germ cells and early embryos supports the hypothesis that serotonin may act as a signalling molecule at early stages of germ line and embryo differentiation via individual receptors present during different stages, when specialized communication systems are not yet developed.
Publisher
Cambridge University Press (CUP)
Subject
Cell Biology,Developmental Biology
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献