The effect of the use of different selective media on the ability to recover salmonellae from seagull faeces

Abstract

SUMMARYSolid media were compared for their ability to recover salmonellae from seagull faecal material after pro-enrichment in buffered peptone water and enrichment in Rappaport's broth. Of the 847 specimens examined 96 were found to be positive for salmonellae. Use of Brilliant Green agar containing sulphamandelate supplement resulted in the detection of salmonellae from each of the 96 samples found to be positive and was the most efficient medium tested. Brilliant Green agar lacking the supplement was the least effective medium, salmonellae being isolated from only 80 samples using this medium.All of the media tested were shown to support the growth of a wide range of salmonella serotypes, althoughSalmonella typhiandS. dublindid not form colonies on those media which contained Brilliant Green. Hynes' modification of deoxycholate citrate agar was shown to be considerably less inhibitory to salmonellae after ageing for four days. Ageing of other media had no significant affect on their ability to support the growth of salmonellae.