The Influence of Temperature, and some other Physical Conditions, on Calf Vaccine

Abstract

1. Temperature influences the rate at which glycerinated lymph deteriorates in accordance with the law of Arrhenius, expressing the effect of temperature upon chemical action; the rate of deterioration increasing three times per 10° rise in temperature.2. Dry powdered calf vaccine in sealed glass capillary tubes still gave rise to typical vesicles after exposure to a temperature of 100°C. for from 5 to 10 minutes.3. The vaccine virus can withstand positive osmosis for 24 hours or more, and negative osmosis for 8 weeks or more, at room temperature.4. It appears doubtful whether the vaccine virus will pass through a Berkefeld V filter even when a stored vaccine emulsion is used for filtration. If any virus does pass through it only does so exceptionally and in small quantities.5. When a glycerinated vaccine emulsion is filtered, white flocculi frequently form in the filtrate. These are at first minute, but subsequently adhere and form a macroscopic sediment. This sediment, when inoculated on calves, does not cause vesiculation.Microscopically these flocculi, in hanging drop preparations, resemble the vaccine bodies described by one or more observers.