Author:
Elzein E. M. E. Abu,Crowther J. R.
Abstract
SUMMARYA simple solid-phase enzyme immunoassay is described for the detection of antibody classes showing activity against foot-and-mouth disease (FMD) virus in bovine sera. The assay achieves a preliminary separation of the specific class of antibody from other serum proteins through immuno-adsorption to class-specific immunoglobulin-coated wells of micro-titre plates. The specific antibody is reacted with FMD virus, which is then detected by an enzyme-labelled anti virus IgG.
Publisher
Cambridge University Press (CUP)
Subject
Public Health, Environmental and Occupational Health,Immunology
Reference8 articles.
1. Further studies of the early antibody in the sera of cattle and guinea pigs infected with foot-and-mouth disease virus;Brown;Journal of Immunology,1964
2. Enzyme-labelled immunosorbent assay techniques in foot-and-mouth disease virus research
3. Radial immunodiffusion and serum neutralization techniques for the assay of antibodies to swine vesicular disease;Golding;Research in Veterinary Science,1976
4. Coupling of enzymes to proteins with glutaraldehyde
5. Evaluation of enzyme-linked immunosorbent assay for quantitation by subclass for bovine antibodies;Sloan;American Journal of Veterinary Research,1978
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