Investigation into rabbit infusion media for the growth ofMycoplasma gallisepticumantigens for inoculation into rabbits

Abstract

SUMMARYSerum cholesterol in rabbits was elevated, by intravenous inoculation of 20 % Tween 80, to amounts thought to have been sufficient to support growth ofMycoplasma gallisepticumwhen 20 % of such serum was added to rabbit infusion broth.However, better growth of the organism was obtained with a supplement of 5 % rabbit serum and commercial cholesterol. Investigation showed that normal rabbit serum may be inhibitory, which would explain these findings. The commercial cholesterol was not antigenic.