A solid-phase enzyme linked immunosorbent assay for the detection of African swine fever virus antigen and antibody

Author:

Wardley R. C.,Abu Elzein E. M. E.,Crowther J. R.,Wilkinson P. J.

Abstract

summaryA solid-phase enzyme-linked immunosorbent assay was developed to measure both African swine fever virus (ASFV) antigen and antibody. Experiments showed it to be reproducible and able to detect limiting antigen concentrations of 50–500 HAD50/ml. The assay was more sensitive than those used at present to detect ASFV antibody and it is suggested that it might be of great diagnostic use in countries where African swine fever has recently appeared.

Publisher

Cambridge University Press (CUP)

Subject

Public Health, Environmental and Occupational Health,Immunology

Reference8 articles.

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2. Solid-phase radioimmunoassay techniques for the detection of African swine fever antigen and antibody

3. Coupling of enzymes to proteins with glutaraldehyde

4. Enzyme-labelled immunosorbent assay techniques in foot-and-mouth disease virus research

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