The diagnostic efficiency of some serological tests for bovine brucellosis

Abstract

SummaryResults obtained from 1887 sera using three serological tests for bovine brucellosis were compared with a serological classification of sera described as the ‘probable infection status’. Sera showing apparent false positive and apparent false negative reactions were identified, and were subjected to supplementary testing as appropriate.The serum agglutination test (SAT) gave 35% apparent false negative reactions and 5% apparent false positives. The complement fixation test (CFT) gave 12% apparent false negative reactions using warm fixation (CFTW) and at least 5% using cold fixation (CFTC). The routine diagnostic system used in Victoria, in which the CFTW is supplemented by the CFTC and the SAT, gave 9% apparent false negative reactions and 2% apparent false positive reactions. The radioimmunoassay gave 1% or 6% apparent false negative reactions, depending the minimum diagnostic value used.Atypical reactions in the CFT sometimes caused difficulties in diagnosis.