Fluid and solid media for isolation of Brucella abortus

Abstract

SUMMARYThe use of a fluid-enrichment technique increased by 10–16% the recovery rate of Brucella abortus from milks of herds positive to a milk ring test. Three solid media for direct cultural techniques were also tested on the same milks. Combinations of these techniques produced a recovery rate of ca. 88% from MRT-positive-herd milks using a mixture of the centrifuged deposit and cream layer as the inoculum as against a recovery rate of ca. 75% using the MRT cream layer as inoculum. B. abortus biotypes 1–5 and 9 were isolated and growth of all the biotypes was supported by all media used.