Location of prophage H90 on the chromosome ofPseudomonas aeruginosastrain PAO

Abstract

SUMMARYProphage H90 has been found to undergo a phenomenon similar to zygotic induction, during conjugal transfer from a lysogenic donor to a non-lysogenic recipient.It has not been possible to demonstrate that the level of infectious centres increases concomitantly with transfer of the prophage. However, the genetic consequence of zygotic induction was observed with regard to decreased recombinant yield of markers distal to the prophage. This latter fact has been exploited in interrupted mating experiments, to locate the prophage at between 5 and 7 min on thePseudomonas aeruginosastrain PAO map. It was further shown by transduction experiments that the prophage does not appear to be linked to clusters of co-transductional markers at the 5 and 7 min locations.