The effect of various physiological conditions on host-controlled restriction inEscherichia coliK(P1)

Abstract

Growth of K(P1) bacteria under conditions which lead to a reduction in the level of nucleases also leads to a reduction of their ability to restrict the growth of λ.C. Experiments designed to estimate the time after adsorption at which restriction takes place indicate that phage DNA is probably restricted by a nuclease while passing through the periplasm.