A semi-automatic technique for conducting many bacterial matings concurrently

Abstract

SUMMARYA technique using microserological equipment is described whereby up to 144 quantitative interrupted bacterial matings may be performed concurrently. The technique is easy to use, accurate and reproducible, and eliminates the need for agar plates. However, it is only suitable for 40–42 °C matings and mating efficiency is approximately threefold lower than in test tubes.