The application of tissue-culture techniques to the chromosomal analysis of Bos taurus

Abstract

Until 1960 all the chromosome counts performed on cells of this species were done on histologically prepared specimens of testicular tissue. In January 1960, Chiarelli et al. published the results of their experiments to grow renal tissue in culture. Their specimens were obtained from male and female calves. They confirmed that 2n = 60 and described the chromosomes as being all acrocentric except the X chromosome, which was submetacentric.The purpose of the work described below was to try to apply the peripheral-blood culture technique of Moorhead to bovine blood, and to attempt to grow bovine muscle cells in a tissue culture.