Genetics of host-controlled restriction and modification inEscherichia coli

Abstract

The sites of mutations affecting host-controlled modification (HCM) have been mapped inE. coliK andE. coliB by conjugation and transduction experiments between mutants. These mutations all map close to theserBlocus on the opposite side to the markerthr. Non-parental HCM has been observed among colonies obtained from Pl transduction experiments between HCM mutants. Control experiments have shown that these non-parental recombinants can not be accounted for by reversion of either parent and must result from recombination between mutants. Several genetic models are suggested which could account for these recombinants and an attempt is made to distinguish between various models by testing for complementation between mutants in a zygotic induction complementation test.