Feeding activities of Cicadulina mbila (Hemiptera: Cicadellidae) on different host-plants

Abstract

AbstractThe probing activities of the most important leafhopper vector of maize streak virus, Cicadulina mbila (Naudé) (Hemiptera: Cicadellidae) were studied on different host-plants. Host preference was determined by giving the insects a choice of six plants, Digitaria sanguinalis, Zea mays, Zea perennis, Paspalum conjugatum, Oryza sativa and Saccharum officinarum (all Gramineae). Cicadulina mbila showed a strong preference for D. sanguinalis. Histological examination of plant tissues for salivary sheaths was used to reveal the preferred tissue. Cicadulina mbila tended to ingest from the phloem of its preferred hosts, and switch to mesophyll or xylem tissue on less preferred hosts. The suitability of each host was assessed by measuring the weight gain and amount of honeydew produced on each host by newly emerged adult females. Both parameters were greatest on the preferred host. Probing behaviour was also monitored electronically by recording the different waveform patterns produced when insects were probing, ingesting or not probing on the selected hosts. Cicadulina mbila probed less frequently, ingested longer and spent less time in non-probing activities on its preferred host, D. sanguinalis, although these responses were similar on maize and sugarcane. Rice induced poor probing responses and altered behaviour. This interpretation of the waveforms was supported by histological examination. As maize streak virus (MSV) is not found in phloem sieve elements, prolonged ingestion from sieve cells would not account for efficient acquisition from maize, which would require ingestion from other types of cells. D. sanguinalis was the host in which most ingestion from the phloem occurred, but it is the poorest source of MSV.