Abstract
AbstractBased on the fact that in haematophagous Diptera, the basal bodies, groups of six to nine specialized epithelial cells in the calyx wall within the ovariolar sheath, become granular after ovulation, a method for rapidly determining parity of the females has been developed. After supravitally staining intact ovaries with fresh neutral red solution in physiological saline (1:3000–1:5000) for one to three minutes, granular basal bodies in parous females can be clearly recognized under a stereo-microscope or a compound microscope with a raised condensor. The advantages of the method are its speed, accuracy and simplicity.
Publisher
Cambridge University Press (CUP)
Subject
Insect Science,Agronomy and Crop Science,General Medicine
Reference50 articles.
1. Yurgenson I.A. & Teplykh V.S. (1966) A technique for determining physiological age of insects. Author's certification No. 195776. (In Russian.)
2. Age Grouping of Female Anopheles Farauti Populations (Diptera: Culicidae) in Papua New Guinea
3. Use of M.I. Sokolova combination method of estimating physiological age in blood-sucking blackflies (Diptera: Simuliidae);Gryaznov;Zoologicheskii Zhurnal,1993
4. The determination of the physiological age of females of Anopheles by changes in the tracheal system of the ovaries;Detinova;Meditsinskaya Parazitologiya i Parasitarnye Bolezni,1945
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