Rho GTPases regulate rhabdom morphology in octopus photoreceptors

Author:

MILLER ARIA M.,RAMIREZ TERESA,ZUNIGA FREDDI I.,OCHOA GINA H.,GRAY SHAUNTE,KELLY SHANNON D.,MATSUMOTO BRIAN,ROBLES LAURA J.

Abstract

In the cephalopod retina, light/dark adaptation is accompanied by a decrease/increase in rhabdom size and redistribution of rhodopsin and retinochrome. Rearrangements in the actin cytoskeleton probably govern changes in rhabdom size by regulating the degradation/formation of rhabdomere microvilli. Photopigment movements may be directed by microtubules present in the outer segment core cytoplasm. We believe that rhodopsin activation by light stimulates Rho and Rac signaling pathways, affecting these cytoskeletal systems and their possible functions in controlling rhabdom morphology and protein movements. In this study, we localized cytoskeletal and signaling proteins in octopus photoreceptors to determine their concurrence between the lighting conditions. We used toxin B fromClostridium difficileto inhibit the activity of Rho/Rac and observed its effect on the location of signaling proteins and actin and tubulin. In both lighting conditions, we found Rho in specific sets of juxtaposed rhabdomeres in embryonic and adult retinas. In the light, Rho and actin were localized along the length of the rhabdomere, but, in the dark, both proteins were absent from a space beneath the inner limiting membrane. Rac colocalized with tubulin in the outer segment core cytoplasm and, like Rho, the two proteins were also absent beneath the inner limiting membrane in the dark. The distribution of actin and Rho was affected by toxin B and, in dark-adapted retinas, actin and Rho distribution was similar to that observed in the light. Our results suggest that the Rho/Rac GTPases are candidates for the regulation of rhabdomere size and protein movements in light-dark-adapted octopus photoreceptors.

Publisher

Cambridge University Press (CUP)

Subject

Sensory Systems,Physiology

Reference70 articles.

1. The rapid synthesis and destruction of photoreceptor membrane by a dinopid spider: a daily cycle

2. Robles, L.J. & Matsumoto, B. (1994).Actin distribution in dark-light adapted octopus retina.Investigative Ophthalmology and Visual Science 35,2130.

3. Blest, A.D. , Stowe, S. , & Eddey, W. (1982).A labile, Ca2+-dependent cytoskeleton in rhabdomeremicrovilli of the blowfly.Cell Tissue Research 223,553–573.

4. McDowell, J.H. , Arendt, A. , Crabb, J.W. , & Smith, W.C. (2004).β-tubulin from retina extracts binds to arrestin.Investigative Ophthalmology and Visual Science 45,E-Abstract 3449.

5. Light-stimulated rhabdom turnover inLimulus ventral photoreceptors maintained in vitro

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