Comparison of microplate esterase assays and immunoassay for identifying insecticide resistant variants of Myzus persicae (Homoptera: Aphididae)

Abstract

AbstractInsecticide resistant Myzus persicae (Sulzer) employ increased esterase activity to detoxify insecticides by hydrolysis and sequestration. The amount of esterase, and hence resistance, in individual aphids can be determined by measuring either overall naphthyl acetate hydrolysis in crude homogenates, or the specific enzyme responsible (E4 or FE4) after electrophoresis or immunological isolation. The ability of a total esterase assay, done in microplates, to discriminate between susceptible (S) aphids and resistant variants (R1, R2) with different amounts of E4/FE4, was compared with the resolving power of the more elaborate immunoassay technique. The immunoassay gave the better discrimination between variants, resolving them all with greater than 95% confidence, with particularly good separation of R1, from S. The microplate assay using crude homogenates, although a poorer discriminator, identified most of the very resistant (R2) aphids, and provided a robust and widely accessible method for broadly representing the resistance of field populations.