Author:
Devonshire Alan L.,Devine Gregor J.,Moores Graham D.
Abstract
AbstractInsecticide resistant Myzus persicae (Sulzer) employ increased esterase activity to detoxify insecticides by hydrolysis and sequestration. The amount of esterase, and hence resistance, in individual aphids can be determined by measuring either overall naphthyl acetate hydrolysis in crude homogenates, or the specific enzyme responsible (E4 or FE4) after electrophoresis or immunological isolation. The ability of a total esterase assay, done in microplates, to discriminate between susceptible (S) aphids and resistant variants (R1, R2) with different amounts of E4/FE4, was compared with the resolving power of the more elaborate immunoassay technique. The immunoassay gave the better discrimination between variants, resolving them all with greater than 95% confidence, with particularly good separation of R1, from S. The microplate assay using crude homogenates, although a poorer discriminator, identified most of the very resistant (R2) aphids, and provided a robust and widely accessible method for broadly representing the resistance of field populations.
Publisher
Cambridge University Press (CUP)
Subject
Insect Science,Agronomy and Crop Science,General Medicine
Cited by
41 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献