Author:
Carew M.E.,Marshall S.E.,Hoffmann A.A.
Abstract
AbstractMolecular approaches for identifying aquatic macroinvertebrate species are increasingly being used but there is ongoing debate about the number of DNA markers needed to differentiate species accurately. Here, we use two mitochondrial genes (cytochrome oxidase I, cytochrome b) and a nuclear gene (carbamoylphosphate synthetase) to differentiate species variation within the taxonomically challenging chironomid genus Procladius from southern Australia, a genus which is important for pollution monitoring. The mitochondrial genes indicated cryptic species that were subsequently linked to morphological variation at the larval and pupal stage. Two species previously described based on morphological criteria were linked to molecular markers, and there was evidence for additional cryptic species. Each genetic marker provided different information, highlighting the importance of considering multiple genes when dissecting taxonomically difficult groups, particularly those used in pollution monitoring.
Publisher
Cambridge University Press (CUP)
Subject
Insect Science,Agronomy and Crop Science,General Medicine
Reference43 articles.
1. Phylogeny of the Genus Chironomus (Diptera) Inferred from DNA Sequences of Mitochondrial Cytochrome b and Cytochrome oxidase I
2. Combining DNA barcoding and morphological analysis to identify specialist floral parasites (Lepidoptera: Coleophoridae: Momphinae:Mompha)
3. Cranston P.S. (2000) Electronic identification guide to the Australian Chironomidae. Avaliable online at http://entomology.ucdavis.edu/chiropage/index.html (accessed 30 August 2010).
4. DNA identification of urban Tanytarsini chironomids (Diptera: Chironomidae);Carew;Journal of the North American Benthological Society,2007a
5. The unholy trinity: taxonomy, species delimitation and DNA barcoding
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