A biochemical and toxicological study of the role of insensitive acetylcholinesterase in organophosphorus resistantBemisia tabaci(Homoptera: Aleyrodidae) from Israel

Abstract

AbstractTwo acetylcholinesterase (AChE) variants, differing in sensitivity to inhibition by the organophosphorus (OP) insecticide paraoxon were identified in a population ofBemisia tabaci(Gennadius) from cotton in Israel using a single insect kinetic microplate assay. Two strains were established, homogeneous for one or other of the two variants, by isolating mated females from the field population onto individual cotton leaves, and testing a proportion of their female offspring to identify their AChE genotype. Polyacrylamide gel electrophoresis of their I-naphthyl butyrate hydrolyzing esterases showed that all insects contained esterase E0 14, which is indicative of B-type whiteflies, although the staining intensity of this band differed. Resistance to the OPs monocrotophos, profenofos and chlorpyrifos in leaf dip bioassays was consistent with the presence of the insensitive AChE. The data also indicated that separate mechanisms conferred resistance to the two pyrethroids cypermethrin and bifenthrin. The former, when used in a mixture with profenofos, was no more toxic than when the OP was used alone, and resistance to the mixture was largely dependent on the presence of the insensitive AChE.