Author:
Brindle R. J.,Stannett P. J.,Cunliffe R. N.
Abstract
SUMMARYA comparison was made between membrane filtration and centrifugation for the isolation ofLegionella pneumophilafrom seeded water samples. Using samples of varying concentration, the optimum speed and time of centrifugation were determined and the relationship between the number of organisms present in the water and the proportion recovered was examined. Following this, sequential routine environmental waters were filtered and centrifuged in parallel.Centrifugation and filtration using nitrocellulose filters were found to be comparable. The optimum speed and time of centrifugation was approximately 6000gfor 10 min. There was a constant proportion of viable organisms recovered irrespective of the concentration in the unspun samples.
Publisher
Cambridge University Press (CUP)
Subject
Infectious Diseases,Epidemiology
Cited by
18 articles.
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