A new multiplex PCR for differential identification ofShigella flexneriandShigella sonneiand detection ofShigellavirulence determinants

Abstract

SUMMARYMost of the multiplex PCR (mPCR) used to identifyShigellado not discriminate betweenShigellaspecies or serotypes. We designed a mPCR to differentiate betweenS. flexneriandS. sonneistrains based on the detection of markers associated with theshepathogenicity island described inShigella. In addition, specific primers were included to detect theShigellavirulence determinants ShET-1 and ShET-2 enterotoxin genes. The analysis of 304Shigellastrains from Chile and 79Shigellastrains from other geographic locations indicated that the mPCR described here detected allShigellaspecies and specifically differentiatedS. flexneriandS. sonnei. The technique was sensitive, reproducible, specific and simple to perform, providing a new tool with the potential to be employed for epidemiological and diagnostic purposes.