A multiplex PCR test to identify four common cattle-adapted Cryptosporidium species

Abstract

SUMMARYCryptosporidiumis a well-known cause of neonatal enteritis in cattle worldwide. Cattle are commonly infected with four different species ofCryptosporidiumbut only one of these,Cryptosporidium parvum, is associated with clinical disease. Identification of species in cases of calf scour can give an indication ifCryptosporidiumis the causative agent or not. In addition,C. parvumis a zoonotic species and so has implications for human health, for this reason it is important to identify the species ofCryptosporidiuminfecting cattle particularly where a farm is implicated in an outbreak of cryptosporidiosis in humans. Here a multiplex PCR test, which can identify the four common cattle-adaptedCryptosporidiumspecies, includingC. parvum, has been developed. This test allows quick and accurate detection ofCryptosporidiumspecies in cattle fecal samples including mixed infections, which could be missed by the more common method of sequencing the same gene.