Performant web-based interactive visualization tool for spatially-resolved transcriptomics experiments

Author:

Sriworarat ChaichontatORCID,Nguyen AnnieORCID,Eagles Nicholas J.ORCID,Collado-Torres LeonardoORCID,Martinowich KeriORCID,Maynard Kristen R.ORCID,Hicks Stephanie C.ORCID

Abstract

Abstract High-resolution and multiplexed imaging techniques are giving us an increasingly detailed observation of a biological system. However, sharing, exploring, and customizing the visualization of large multidimensional images can be a challenge. Here, we introduce Samui, a performant and interactive image visualization tool that runs completely in the web browser. Samui is specifically designed for fast image visualization and annotation and enables users to browse through large images and their selected features within seconds of receiving a link. We demonstrate the broad utility of Samui with images generated with two platforms: Vizgen MERFISH and 10x Genomics Visium Spatial Gene Expression. Samui along with example datasets is available at https://samuibrowser.com.

Funder

Chan Zuckerberg Initiative

National Institute of Mental Health

Publisher

Cambridge University Press (CUP)

Subject

General Earth and Planetary Sciences,General Environmental Science

Reference28 articles.

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2. 28. Vizgen MERFISH Mouse Receptor Map. https://info.vizgen.com/mouse-brain-data (accessed 10 November 2022).

3. 11. Spatial Gene Expression – 10x Genomics. https://www.10xgenomics.com/products/spatial-gene-expression (accessed 21 December 2022).

4. 27. Data Release Program | Vizgen. https://vizgen.com/data-release-program/ (accessed 21 December 2022).

5. Spatial transcriptome profiling by MERFISH reveals subcellular RNA compartmentalization and cell cycle-dependent gene expression

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