The metabolic profile of adultFasciola hepaticaobtained from rafoxanide-treated sheep

Abstract

SummarySheep infected with adultFasciola hepaticawere drenched with rafoxanide. At 4, 8, 16 and 24 h after drenching the sheep were killed and the flukes removed, washed and rapidly frozen in liquid nitrogen. The content of key metabolites in the fermentation pathway were determined and compared with those in controlF. hepatica, whose hosts were not treated with rafoxanide.Rafoxanide decreased glycogen, malate, NADH and ATP levels. The level of other metabolites in the pathway increased for the first 8–16 h after rafoxanide treatment. The marked decrease in ATP and glycogen, and the increase in total [NAD+]/[NADH] and [oxaloacetate]/[malate], together with the changed content of other metabolites, led to the conclusion that the mode of action of rafoxanide againstF. hepatica in vivois by uncoupling oxidative phosphorylation.