Author:
WU Z.,NAGANO I.,TAKAHASHI Y.
Abstract
Diagnostic PCR primers for Trichinella were constructed.
Twelve pairs of primers were designed based on the sequences
of random amplified polymorphic DNA, and 4 pairs of primers were designed
based on the reported sequences of
complementary DNA encoding excretory–secretory glycoproteins.
With these primers, 31 samples of DNA from different
strains of Trichinella including 5 species (Trichinella spiralis,
Trichinella britovi, Trichinella nativa, Trichinella
nelsoni and
Trichinella pseudospiralis) and 3 phenotypes of uncertain
taxonomic level (Trichinella T5, T6 and T8) were tested with
PCR. Genus Trichinella can be identified by 4 different primer
pairs (SB147D, SB372A, SB153, or Ts43). Trichinella
spiralis can be identified by the presence of a 673 bp amplicon in
PCR
with the primer pair SB147B. Trichinella nelsoni
can be identified using primer pair SB147F or by the presence of 673 bp
and
ca. 380 bp amplicon in PCR with the primer
pair SB147B. Trichinella pseudospiralis can be identified by
2 primer pairs (SB147E or SB372B). Trichinella T5 can be
identified by the primer pair SB147G. Trichinella T8 can be
identified by its positivity by the primer pair SB147C and
its negativity by the primer pair SB372C. A group of Trichinella
species (T. britovi, T. nativa and Trichinella
T6) can be
identified by the primer pair SB372C.
Publisher
Cambridge University Press (CUP)
Subject
Infectious Diseases,Animal Science and Zoology,Parasitology
Cited by
37 articles.
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