Author:
FREITAS M. A. R.,VIANNA E. N.,MARTINS A. S.,SILVA E. F.,PESQUERO J. L.,GOMES M. A.
Abstract
In this study, a single-step duplex polymerase chain reaction procedure was developed for rapid, specific and sensitive identification ofEntamoeba histolyticaand for its diagnostic differentiation fromE. dispar. Specific oligonucleotide primers were combined for the amplification of a cysteine proteinase 5 gene target sequence of 242 bp, present only inE. histolytica. Additionally, another oligonucleotide primer pair for both theE. histolyticaandE. disparactin gene target of 300 bp was designed to amplify only from amoebae DNA. The PCR developed was specific and efficiently identified and differentiated these parasites from each other in either cultured parasites or from stool material.
Publisher
Cambridge University Press (CUP)
Subject
Infectious Diseases,Animal Science and Zoology,Parasitology
Cited by
19 articles.
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