Abstract
The effect of digestion in trypsin and acid pepsin on
Toxoplasma gondii tachyzoites and bradyzoites was re-evaluated
because of recent use of this method to distinguish tachyzoites
from bradyzoites. Toxoplasma gondii tachyzoites survived
better in 0·5% trypsin solution for 1 h than in 1·0%
solution, and occasionally survived for 2 h in acid pepsin solution.
Extracellular tachyzoites ([ges ]1000) were also infectious orally
to mice and cats. Bradyzoites survived equally in trypsin and
acid pepsin solutions but the digestion of brain tissue in 0·5%
trypsin solution was better than in acid pepsin solution.
The resistance to digestion in acid pepsin solution is not a
reliable method to distinguish tachyzoites from bradyzoites.
Publisher
Cambridge University Press (CUP)
Subject
Infectious Diseases,Animal Science and Zoology,Parasitology
Cited by
91 articles.
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