Isolation and characterization of polysomes fromTrypanosoma brucei

Abstract

SUMMARYThe isolation of polysomes in bulk from bloodstream forms ofTrypanosoma bruceiis described. The polysomes are active inin vitroprotein synthesis in the presence or absence of initiation inhibitors. Nascent variant surface antigen (VSA) has been detected on these polysomes using purified radio-iodinated antibody. EDTA-induced ribosomal sub-units and their large rRNA's are characterized. The 26S rRNA is nicked to produce 2 molecules which are both smaller than the 195 rRNA of the small sub-unit which is larger than that found in the majority of eukaryotic small sub-units.