The role of surface glycoconjugates in Leishmania midgut attachment examined by competitive binding assays and experimental development in sand flies

Author:

JECNA LUCIE,DOSTALOVA ANNA,WILSON RAY,SEBLOVA VERONIKA,CHANG KWANG-POO,BATES PAUL A.,VOLF PETR

Abstract

SUMMARYBinding of promastigotes to the sand fly midgut epithelium is regarded as an essential part of the Leishmania life cycle in the vector. Among Leishmania surface molecules putatively involved in attachment to the sand fly midgut, two GPI-anchored molecules are the most prominent: lipophosphoglycan (LPG) and promastigote surface protease gp63. In this work, we examined midgut attachment of Leishmania lines mutated in GPI-anchored molecules and compared results from 2 different techniques: in vivo development in sand flies and in vitro competitive binding assays using fluorescently labelled parasites. In combination with previous studies, our data provide additional support for (1) an LPG-independent parasite-binding mechanism of Leishmania major within the midgut of the permissive vector Phlebotomus perniciosus, and provide strong support for (2) the crucial role of L. major LPG in specific vector Phlebotomus papatasi, and (3) a role for Leishmania amazonensis gp63 in Lutzomyia longipalpis midgut binding. Moreover, our results suggest a critical role for GPI-anchored proteins and gp63 in Leishmania mexicana attachment to L. longipalpis midguts, as the wild type (WT) line accounted for over 99% of bound parasites.

Publisher

Cambridge University Press (CUP)

Subject

Infectious Diseases,Animal Science and Zoology,Parasitology

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1. Role of Virulence Factors of Trypanosomatids in the Insect Vector and Putative Genetic Events Involved in Surface Protein Diversity;Frontiers in Cellular and Infection Microbiology;2022-04-28

2. Leishmaniasis: Molecular Aspects of Parasite Dimorphic Forms Life Cycle;Leishmaniasis - General Aspects of a Stigmatized Disease;2022-04-13

3. Structural characterization of a galectin isolated from the marine sponge Chondrilla caribensis with leishmanicidal potential;Biochimica et Biophysica Acta (BBA) - General Subjects;2021-12

4. Lutzomyia longipalpis: an update on this sand fly vector;Anais da Academia Brasileira de Ciências;2021

5. Lutzomyia longipalpis: an update on this sand fly vector;Anais da Academia Brasileira de Ciências;2021

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