Author:
COUZINET S.,DUBREMETZ J. F.,BUZONI-GATEL D.,JEMINET G.,PRENSIER G.
Abstract
We have studied the activity of the polyether ionophorous antibiotic monensin against the cyst form (bradyzoite) of
Toxoplasma gondii. The experiments were conducted in vitro using 2 methods; cysts produced either in mice or in cell
culture were exposed to monensin in vitro, and the infectivity of the parasites was then assessed in vivo or in vitro. The
data obtained from these 2 systems of evaluation showed that monensin inhibits the infectivity and the viability of the
bradyzoites. Its activity was time and concentration dependent. The first effects were observed at very low drug
concentrations (i.e. 0·0001 μg/ml). Immunofluorescence and electron microscopy analysis showed significant cytological
alterations of the monensin-treated bradyzoites: they were swollen, had a large number of vacuoles in their cytoplasm and
were found lysed at higher concentrations in ionophore.
Publisher
Cambridge University Press (CUP)
Subject
Infectious Diseases,Animal Science and Zoology,Parasitology
Cited by
28 articles.
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