Author:
Philipp M.,Gómez-Priego A.,Parkhouse R. M. E.,Davies M. W.,Clark N. W. T.,Ogilvie Bridget M.,Beltrán-Hernández F.
Abstract
SummaryAdult Onchocerca volvulus worms obtained by enzyme digestion from nodules of infected Mexicans were radio-isotope labelled by the chloramine-T or Bolton–Hunter methods. No antigenic determinants were detected in extracts of worms labelled by the chloramine-T method but 3 antigens were detected in extracts of the Bolton–Hunter labelled worms. Two were present in such small amounts that it was impractical to investigate them further, but a major component of mol. wt 20 kDa was purified by gel filtration and used in a serological survey of inhabitants of villages in Southern Mexico. Using the 20 kDa antigen, which is superficially located on both sexes of O. volvulus, sera from both non-endemic and endemic regions were analysed by radio-immunoprecipitation of this antigen. In Southern Mexico, the average sensitivity of the test was 92%, and the specificity 98%. Whilst the 20 kDa antigen did not detect antibodies in the sera of Trinidadians infected with Wuncheria bancrofti or Mansonella ozzardi, this antigen detected high levels of antibodies in Indians exposed to W. bancrofti.
Publisher
Cambridge University Press (CUP)
Subject
Infectious Diseases,Animal Science and Zoology,Parasitology
Reference25 articles.
1. A Review of Immunologic Methods for the Diagnosis of Filariasis
2. Immunological diagnosis of human filariasis: Present possibilities, difficulties and limitations;Ambroise-Thomas;Acta Tropica,1974
3. Changing proteins on the surface of a parasitic nematode
4. Immunoglobulins and complement in sera from patients with various parasitic infections;Higashi;Indian Journal of Medical Research,1971
5. Isolation of living adult Onchocerca volvulus from nodules;Schulz-Key;Tropenmedizin und Parasitologie,1977
Cited by
43 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献