An electron microscope study of the tegument of the metacercaria and adult of Leucochloridiomorpha constantiae (Trematoda: Brachylaemidae)

Abstract

The tegumentary ultrastructure of Leucochloridiomorpha constantiae metacercariae and adults has been described. A filamentous glycocalyx invests the tegument of metacercariae and large numbers of biconcave, disk-shaped secretion vesicles are found in its outer zone, cytoplasmic bridges and cytons. Mitochondria within the outer tegumentary zone of metacercariae are restricted to its basal half. The transformation of metacercariae to adults involves the loss of the glycocalyx, a decrease in the number of disk-shaped secretion vesicles, a more uniform distribution of mitochondria through the outer tegumentary zone, an apparent degeneration of cytons, a thinning of the fibrous basal lamina and a decrease in the number of visible cytoplasmic bridges traversing it. The significance of these events for a metacercaria which does not encyst within the molluscan host is discussed. In addition, crystalline, spine-like inclusions in the outer tegumentary zone of adult L. constantiae are described and their possible function discussed.