Glucose and pyruvate catabolism inLitomosoides carinii

Abstract

SummaryThe filarial wormLitomosoides cariniishowed a rapid uptake of glucose duringin vitroincubation. This uptake proceeded linearly with time, and was significantly higher under aerobic compared to anoxic conditions. Under an atmosphere of nitrogen the worms converted glucose almost quantitatively to lactate, whereas in the presence of oxygen appreciable quantities of acetate, acetoin and CO2, in addition to lactate, were formed. Although aerobically only 73% of the carbohydrate carbon could be accounted for by the latter products as well as by a net glycogen synthesis, attempts to identify other compounds presumed to be derived from glucose metabolism have been unsuccessful. The complete sequence of the glycolytic enzymes was detected in particulate-free cytosolic extracts of the filarial worm. With the exception of 6-phosphofructokinase, all glycolytic enzyme activities were considerably higher than those reported for rat liver. In addition,L. cariniipossesses the entire set of enzymes catalysing the eight successive reaction steps of the tricarboxylic acid cycle. On a mitochondrial protein basis, the specific activities of these enzymes were similar to those present in rat liver. Various enzymatic activities of the mitochondrial respiratory chain were detected in the parasite. These include low levels of NADH and cytochrome c oxidases, but a high activity value for NADH dehydrogenase. Cell-free extracts and the mitochondrial fraction of the worms were found to exhibit an enzyme capable of catalysing the decarboxylation of pyruvate. Since this activity was stimulated 5- to 20-fold by the cofactors known to be required by the pyruvate dehydrogenase complex of other animal cells, pyruvate decarboxylation and thus acetate formation in the parasite may be mediated by an enzyme similar to, or identical with, the pyruvate dehydrogenase system. Isotopic carbon balance studies and experiments in which substrates specifically labelled with14C were employed showed that substrate carbon can to some extent enter into respiratory CO2. From these and the enzymatic analyses it is suggested that complete oxidation of carbon substrate may be of relevance as an energy-conserving pathway in the filarial worm.