Author:
DAHLGREN STINA S.,GJERDE BJØRN
Abstract
SUMMARYMuscle tissue from 37 red deer from Norway was examined for sarcocysts. Sarcocysts from 2 reindeer were obtained for comparative studies. Cysts were excised and morphologically classified by light microscopy, scanning electron microscopy, and DNA sequence analysis. FiveSarcocystisspecies,Sarcocystis hjortin. sp.,Sarcocystis hardangeri, Sarcocystis ovalis, Sarcocystis rangiferi, andSarcocystis tarandi, were found. All 5 species have previously been identified from either reindeer or moose by their sarcocyst morphology and/or ssu rRNA gene sequence.S. hjortiwas the most prevalent species. Multiple variants of the ssu rRNA gene and the first internal transcribed spacer were found inS. rangiferiandS. tarandifrom both red deer and reindeer. Phylogenetic analyses indicated thatS. tarandioccurs in both red deer and reindeer, but it could not be clearly demonstrated whether the sequence variation withinS. rangiferibetween hosts was due to different paralogues or/and different species. DNA sequencing was necessary for definitive species identification, since the hair-like protrusions on the cysts ofS. hjortiwere not always recognizable by light microscopy and since different cervids harbourSarcocystisspecies with highly similar cyst morphology of which at least some are not intermediate host specific.
Publisher
Cambridge University Press (CUP)
Subject
Infectious Diseases,Animal Science and Zoology,Parasitology
Reference63 articles.
1. Phylogeny and sequence variability of the Sarcocystis singaporensis Zaman and Colley, (1975) 1976 ssrDNA;Slapeta;Parasitology Research,2002
2. Evidence to support horses as natural intermediate hosts for Sarcocystis neurona
3. The current status of the small subunit rRNA phylogeny of the coccidia (Sporozoa)
4. Ultrastruktur der Cystenwand der Sarkosporidien des Rehes (Capreolus capreolus L.)
5. Licht- und elektronenmikroskopische Untersuchungen an Cysten von Sarcocystis fusiformis in der Muskulatur von Kälbern nach experimenteller Infektion mit Oocysten und Sporocysten von Isospora hominis Raillet et Lucet, 1891;Mehlhorn;Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene, I. Abteilung Originale A,1975
Cited by
51 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献