Author:
ZHU X. Q.,JACOBS D. E.,CHILTON N. B.,SANI R. A.,CHENG N. A. B. Y.,GASSER R. B.
Abstract
The ascaridoid nematode of cats from Kuala Lumpur, Malaysia,
previously identified morphologically as Toxocara canis,
was characterized using a molecular approach. The nuclear ribosomal
DNA (rDNA) region spanning the first internal
transcribed spacer (ITS-1), the 5·8S gene and the second
internal transcribed spacer (ITS-2) was amplified and sequenced.
The sequences for the parasite from Malaysian cats were compared with
those for T. canis and T. cati. The sequence data
showed that this taxon was genetically more similar to T. cati
than to T. canis in the ITS-1, 5·8S and ITS-2. Differences
in the ITS-1 and ITS-2 sequences between the taxa
(9·4–26·1%) were markedly higher than variation between
samples
within T. canis and T. cati (0–2·9%).
The sequence data demonstrate that the parasite from Malaysian cats is
neither T.
canis nor T. cati and indicate that it is a distinct
species. Based on these data, PCR-linked restriction fragment length
polymorphism (RFLP) and single-strand conformation polymorphism (SSCP)
methods
were employed for the unequivocal differentiation of the
Toxocara variant from T. canis and T. cati.
These methods should provide valuable tools
for studying the life-cycle, transmission pattern(s) and zoonotic potential
of this parasite.
Publisher
Cambridge University Press (CUP)
Subject
Infectious Diseases,Animal Science and Zoology,Parasitology
Cited by
89 articles.
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