Author:
ZAKAI H. A.,CHANCE M. L.,BATES P. A.
Abstract
Promastigotes of Leishmania braziliensis, L. donovani,
L. major and L. mexicana recently derived from tissue
amastigotes
were cultured in Schneider's Drosophila medium supplemented
with
20% (v/v) heat-inactivated foetal calf serum and 25 μg gentamicin
sulfate/ml at pH 5·5. These cultures produced more metacyclic
promastigotes in their stationary-phase
populations than others cultured at pH 7·0. Metacyclic promastigotes
possessed a short ([les ]8 μm) and narrow ([les ]1·5 μm)
cell body with a flagellum twice or more the length of the cell body.
Promastigotes from acidic cultures were more resistant
to complement-mediated lysis and more infective in vivo than
those grown at neutral pH. These results demonstrate that
induction of metacyclogenesis by acidic pH is a response conserved across
a variety of species of Leishmania.
Publisher
Cambridge University Press (CUP)
Subject
Infectious Diseases,Animal Science and Zoology,Parasitology
Cited by
79 articles.
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