Author:
Franek Michal,Suchánková Jana,Sehnalová Petra,Krejčí Jana,Legartová Soňa,Kozubek Stanislav,Večeřa Josef,Sorokin Dmitry V.,Bártová Eva
Abstract
AbstractStudies on fixed samples or genome-wide analyses of nuclear processes are useful for generating snapshots of a cell population at a particular time point. However, these experimental approaches do not provide information at the single-cell level. Genome-wide studies cannot assess variability between individual cells that are culturedin vitroor originate from different pathological stages. Immunohistochemistry and immunofluorescence are fundamental experimental approaches in clinical laboratories and are also widely used in basic research. However, the fixation procedure may generate artifacts and prevents monitoring of the dynamics of nuclear processes. Therefore, live-cell imaging is critical for studying the kinetics of basic nuclear events, such as DNA replication, transcription, splicing, and DNA repair. This review is focused on the advanced microscopy analyses of the cells, with a particular focus on live cells. We note some methodological innovations and new options for microscope systems that can also be used to study tissue sections. Cornerstone methods for the biophysical research of living cells, such as fluorescence recovery after photobleaching and fluorescence resonance energy transfer, are also discussed, as are studies on the effects of radiation at the individual cellular level.
Publisher
Cambridge University Press (CUP)
Reference90 articles.
1. Selective plane illumination microscopy techniques in developmental biology
2. Live cell spinning disk microscopy;Graf;Adv Biochem Eng Biotechnol,2005
3. 3D imaging of Sox2 enhancer clusters in embryonic stem cells
4. Considerations on a laser-scanning-microscope with high resolution and depth of field;Cremer;Microsc Acta,1978
5. Post-Translational Modifications of Histones in Human Sperm
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