Abstract
AbstractThe detailed kinetics study of erythrocyte deformability is useful for the early diagnosis of blood diseases and for monitoring the blood rheology. Present solutions for a three-dimensional (3D) reconstruction of erythrocytes has a limited potential. This study aimed to use erythrocyte transmission electron images (ETIs) to evaluate the morphological relationship between adjacent ETIs and generate erythrocytes 3D model. First, ultrathin serial sections of skeletal muscle tissue were obtained using an ultramicrotome. Further, the set of ETIs in a capillary were captured by transmission electron microscopy. The images were aligned by translations and rotations using custom software to optimize the morphological relationship between adjacent ETIs. These coordinate transformations exploit the unique principal axis of inertia of each image to define the body coordinate system and hence provide the means to accurately reconnect the adjacent ETIs. The sum of the distances between the corresponding points on the boundary of adjacent ETIs was minimized and, further, was optimized by using physiological relationship between the adjacent ETIs. The analysis allowed to define precise virtual relationship between the adjacent erythrocytes. Finally, extracted erythrocytes’ cross-section images allowed to generate 3D model of the erythrocytes.
Publisher
Cambridge University Press (CUP)
Reference24 articles.
1. 3D visualization and quantitative analysis of human erythrocyte phagocytosis
2. Carata L , Shao D , Hadwiger M Groller E (2011) Improving the visualization of electron-microscopy data through optical flow interpolation,” Proceedings of the 27th Spring Conference on Computer Graphics, April 28–30, 2011, Viničné, Slovak Republic, pp. 1–8.
3. Fan YY Sun YY (2017) A method for human identification based on bone in vivo and a computer readable medium (Patent number: China#20170550615.5).
4. On the Progress of Scanning Transmission Electron Microscopy (STEM) Imaging in a Scanning Electron Microscope