Evaluation ofVcAVNTR as a strain-typing and phylogeny study method ofVibrio choleraestrains

Abstract

SUMMARYThis study attempted to examine the relatedness between RAPD–PCR, PFGE andVcAVNTR results with those of conventional phage typing ofV. choleraestrains and to evaluateVcAVNTR as an indispensable molecular-typing tool that accomplishes the urgent need for effective epidemiological surveillance. All the O1 El Tor strains were predominantly clustered into phage type T27 with the new phage-typing scheme. Using RAPD–PCR, a total of 69 O1 El Tor strains were grouped under 16 different electrophoretic patterns. A total of 33 pulsotypes were identified in these strains by PFGE.VcAVNTR revealed highVcApolymorphism in allV. choleraestrains incorporated in this study. Our results underline the considerable potential ofVcAVNTR analysis as a tool for molecular typing ofV. cholerae.