Author:
DALSGAARD A.,SERICHANTALERGS O.,FORSLUND A.,PITARANGSI C.,ECHEVERRIA P.
Abstract
Seventy clinical strains of Vibrio cholerae O1 isolated
from 1982–96 in Samutsakorn, a port
city 30 km southwest of Bangkok where cholera occurs at low levels with
regular seasonality,
were characterized to investigate if there were any differences among the
O1 strains isolated
before, during and after the O139 epidemic. Pulsed-field gel electrophoresis
(PFGE) typing,
ribotyping and southern blot hybridization with a cholera toxin probe (CT
genotyping)
demonstrated several genotypes among O1 strains isolated
before the emergence of V. cholerae
O139. However, O1 strains isolated during and after the advent of O139
showed identical
ribotypes which were distinctly different from the types
identified in strains isolated before the
emergence of O139. Ribotypes identified in strains during and after the
advent of O139 were
also demonstrated by O1 strains isolated immediately before the emergence
of O139.
Considering the seasonality of cholera in Samutsakorn, the identical ribotype
and CT genotype
and the closely related PFGE types shown by all O1 strains isolated during
and after the
appearance of O139 is remarkable and suggest that the
V. cholerae O1 strain may reemerge
from an environmental source. A subgroup of V. cholerae O1 strains
isolated before the
emergence of the O139 epidemic had a ribotype identical to a type demonstrated
by O139
strains isolated in Thailand. Our results support similar findings in Bangladesh
and India that
a distinct O1 strain appeared during the O139 epidemic. However, compared
with the apparent
identical strain which replaced O139 in Bangladesh and India, the emerged
O1 strain in
Samutsakorn showed a different ribotype and CT genotype.
Publisher
Cambridge University Press (CUP)
Subject
Infectious Diseases,Epidemiology
Cited by
20 articles.
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