Response of Calli and Seedlings of Tomato (Lycopersicon esculentum) Cultivars to Metribuzin

Abstract

Hypocotyl callus cultures were established from six tomato (Lycopersicon esculentum L.) cultivars that exhibited differential tolerance to preemergence and postemergence metribuzin [4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5(4H)-one] at 0.5 kg ai/ha in the greenhouse. Metribuzin in a low sucrose growth medium inhibited callus growth, but calli were not highly sensitive to the herbicide Differences between cultivars in the response of calli to 50 ppm metribuzin were observed. The degree of tolerance of tomato cultivars to postemergence metribuzin at 0.5 kg/ha was similar to the degree of tolerance of their hypocotyl calli to 50 ppm metribuzin in the growth medium.