Effect of R-25788 on EPTC Metabolism in Corn(Zea mays)

Abstract

R-25788(N,N-diallyl-2,2-dichloroacetamide) had no effect on14CO2evolution from carbonyl-14C-EPTC(S-ethyl dipropylthiocarbamate) treated corn(Zea maysL. ‘inbred Oh551’). Treatment with 1.0 ppmw R-25788 increased production of water-soluble and unextractable products from EPTC and increased the rate of disappearance of organic-soluble radioactivity. R-25788 increased levels of reduced and oxidized glutathione in corn roots. Pretreatment of corn in nutrient solution with R-25788 had no effect on glutathione synthetase activity in root tissue, but R-25788 at 5.0 and 500.0 nM showed an in vitro stimulation of the enzyme.