A selective oleic acid albumin agar medium for the cultivation ofMycobacterium bovis

Abstract

SUMMARYThe modification of Middlebrook's 7H11 oleic acid albumin agar medium by the addition of fresh bovine serum and lysed sheep red cells to encourage growth ofMycobacterium bovisis described. The improved medium was made selective by the addition of antibiotics and a comparative trial of this medium and the guineapig test in the isolation ofM. bovisfrom badger tissues is reported. A close agreement between the two tests was found; the guinea-pig test detected 95% of all isolations and culture detected 91%.