An evaluation of strontium chloride, Rappaport and strontium selenite enrichment for the isolation of salmonellas from man, animals, meat products and abattoir effluents

Abstract

SUMMARYStrontium chloride enrichment broth was found to be comparable to Rappaport broth for the recovery of a wide range ofSalmonellaserotypes from man, animals, meat products and effluents. With the exception of cloacal samples from reptiles, both procedures were superior to selenite F.The performance of strontium chloride M and selenite F enrichment was improved when effluent samples were incubated at 43° C.Strontium chloride M and Rappaport enrichment were superior to selenite F for the isolation ofArizonaspecies from reptiles.Strontium chloride B, strontium selenite and Rappaport broths were found suitable for the isolation of multipleSalmonellaserotypes from sea water contaminated with abattoir effluents. The strontium chloride B and strontium selenite enrichment media were superior to Rappaport broth when samples were incubated at 43° C.Modified bismuth sulphite agar was found superior to Salmonella-Shigella agar as a solid subculture medium.The investigation of a food poisoning outbreak due toSalmonella typhimuriumphage type 21 is reported.The significance of the choice of sampling and isolation techniques in salmon-ellosis in man and animals is discussed.