Speciation, serotyping, antimicrobial sensitivity and plasmid content of Proteeae from the environment of calf-rearing units in South West England

Abstract

SUMMARYA survey was undertaken of the occurrence, serotype, antimicrobial sensitivity and plasmid content of members of the tribe Proteeae in the environment of two calf-rearing units in the county of Avon in South West England. Examples of the following species were found:Proteus mirabilis, Prot. vulgaris, Prot. vulgarisBiogroup 2,Morganella morganii, Providencia stuartii, Prov. alcalifaciensandProv. rettgeri. A wide range of sero types was found, many having been previously reported from nosocomial isolates. A total of 15% of isolates carried plasmids; six pairs of isolates were identified which had identical sero typesbut different patterns of plasmid carriage. The antimicrobial sensitivity of the isolates was generally similar to isolates of Proteeae from humans. Although no truly aminoglycosideresistantisolates were found, some isolates ofProv. stuartiiandProv. rettgerihad MIC's higher than the other isolates to gentamicin and netilmicin, suggesting the presence of lowlevels of the enzyme AAC 2′. The study demonstrates that there is a considerable diversityof species and types of Proteeae associated with calves and their environment. It seems likely that a potential cause of colonization of the human gut by Proteeae is the consumption of meat.